Acqualagna is a comune (municipality) in the Province of Pesaro e Urbino, in the Italian region of Marche. It lies about 70 kilometres (43 mi) west of Ancona and about 40 kilometres (25 mi) southwest of Pesaro. As of 31 December 2004, its population was 4,304, and the comune covers an area of 50.8 square kilometres (19.6 sq mi).
Acqualagna is one of the main centres in Italy for truffles. The town also sits at the confluence of two rivers: the Candigliano is joined by the Burano in the vicinity of Acqualagna.