Ayoó de Vidriales is a municipality in the province of Zamora, within the autonomous community of Castile and León, Spain. It lies in the northwestern part of the country, in the region of the Vidriales valley, from which it takes part of its name.
The municipality is small and rural, with a recorded population of 397 inhabitants according to the 2009 census (INE). Like many villages in this part of Zamora, it is characterised by a low and gradually declining population base.
Its administrative status as a municipality places it under the local governance structures of the province of Zamora and the wider Castile and León region. The surrounding area is part of the broader Leonese countryside, known for its agricultural landscape and historic small settlements.