Amusquillo is a municipality located in the province of Valladolid, in the autonomous community of Castile and León, Spain. It is one of the many small rural settlements that make up this region of north-central Spain.
According to the 2004 census conducted by the Instituto Nacional de Estadística (INE), the municipality had a population of 161 inhabitants. This small population figure reflects the character of Amusquillo as a minor, sparsely populated municipality typical of the surrounding countryside.