Castilleja de Guzmán is a municipality in the province of Seville, in the autonomous community of Andalusia, Spain. It lies within the comarca of the Aljarafe, the elevated area to the west of Seville, and forms part of the wider metropolitan area of the provincial capital.
According to the 2005 census conducted by the Instituto Nacional de Estadística (INE), the municipality had a population of 2,409 inhabitants. The town is one of several settlements in the Aljarafe whose names begin with "Castilleja," a group that also includes Castilleja de la Cuesta and Castilleja del Campo.