Tolhuin is a town in the province of Tierra del Fuego, Argentina. It is located on the eastern shore of Lake Fagnano, in the southern part of the Isla Grande de Tierra del Fuego.
With 9,879 inhabitants as per the 2022 census, Tolhuin is the third largest settlement on the Argentine side of Tierra del Fuego, after Ushuaia and Río Grande.