Villa Regina is a city in the General Roca Department of Río Negro Province, Argentina. It lies in the Upper Río Negro Valley, a fruit-growing region of northern Patagonia, and is connected to the other towns of the valley by National Route 22.
The city is one of the settlements of the Alto Valle del Río Negro, an area shaped by irrigation from the Río Negro and known for intensive agriculture, particularly apples and pears. Villa Regina shares this economic and geographic setting with neighbouring towns along the same route.