Owensboro is a city located in Daviess County, Kentucky, in the United States. It serves as the county seat and is the principal city of the Owensboro metropolitan area. The city is situated on U.S. Route 60 and Interstate 165, approximately 107 miles (172 km) southwest of Louisville.
As of the 2020 census, Owensboro had a population of 60,183. It is the fourth-most populous city in Kentucky. The surrounding metropolitan area had an estimated population of 116,506 as of 2018, making it the sixth largest metropolitan area in the state and the seventh largest population center when including micropolitan areas.