Santa Giustina in Colle is a comune (municipality) located in the Province of Padua within the Veneto region of Italy. It is situated approximately 35 kilometres (22 mi) northwest of Venice and about 15 kilometres (9 mi) north of Padua.
The municipality shares borders with several neighboring areas, including Campo San Martino, Camposampiero, Castelfranco Veneto, Loreggia, San Giorgio delle Pertiche, San Martino di Lupari, and Villa del Conte.