Mespelbrunn is a municipality located in the Aschaffenburg district within the Regierungsbezirk of Lower Franconia (Unterfranken) in Bavaria, Germany. It serves as a member of the Verwaltungsgemeinschaft of Mespelbrunn, which has its seat in Heimbuchenthal.
The town is situated in the Bavarian region and functions as part of the local administrative structure of Lower Franconia.